| LAP TGF-β1

Transforming growth factor beta 1 or TGF-β1 is a polypeptide member of the transforming growth factor beta superfamily of cytokines. It is a secreted protein that performs many cellular functions, including the control of cell growth, cell proliferation, cell differentiation, and apoptosis.TGF-β is a multifunctional set of peptides that controls proliferation, differentiation, and other functions in many cell types.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (pmol/L)	50	25	12.5	6.25	3.12	1.56	0.78	0
OD	2.33	1.55	1.02	0.64	0.38	0.26	0.18	0.13
Corrected OD	2.2	1.42	0.89	0.51	0.25	0.13	0.05	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human LAP TGF-β1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	83-113	94
Plasma(n=8)	88-105	98
Cell Culture Supernates(n=8)	81-114	100

 

| Linearity

Samples were spiked with high concentrations of Human LAP TGF-β1 and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	83-113	88-106	83-94
	Average (%)	99	94	86
1 : 4	Range (%)	90-111	86-99	83-102
	Average (%)	103	90	91